Isolation of All Tryptic Peptides from the Aminoethylated β Polypeptide Chain of Adult Hemoglobin from Japanese Monkey (Macaca fuscata fuscata) and their Amino Acid Compositions
نویسنده
چکیده
Hemoglobin prepared from red blood cells of adult Japanese Monkeys (Macaca fuscata fuscata) was removed heme so that globin was obtained. The • @ globin • @ was • @ separated • @into • @ ƒ¿ • @ and ƒÀ polypeptide • @ chains • @ by • @ countercurrent distribution with the system of sec-butanol containing trichroloacetic acid, propionic • @ acid, • @and • @water. • @ The,ƒÀ • @ polypeptide • @ chain • @was • @aminoethylated • @ and hydrolyzed with trypsin. The tryptic peptides were isolated and purified by column chromatography, paper chromatography, and high voltage paper electrophorsis. Analyses of amino acid compositions of these peptides were performed after HCl hydrolysis. In comparison with human hemoglobin A, Japanese monkey hemoglobin turned out to be different at at least seven positions of the five peptides from • @the ƒÀ polypeptide • @ chain. • @ Similarly, • @ in • @ comparison • @ with • @rhesus • @ monkey hemoglobin, only one difference in the amino acid composition could be found between rhesus monkey (macaca mulatta) and Japanese monkey which belong to the common genus.
منابع مشابه
Isolation of all tryptic peptides from the aminoethylated beta polypeptide chain of adult hemoglobin from Japanese monkey (Macaca fuscata fuscata) and their amino acid compositions.
متن کامل
Isolation of All Soluble Tryptic Peptides from the α Polypeptide Chain of Adult Hemoglobin from Japanese Monkey (Macaca fuscata fuscata) and their Amino Acid Compositions
متن کامل
Isolation of all soluble tryptic peptides from the alpha polypeptide chain of adult hemoglobin from Japanese monkey (Macaca fuscata fuscata) and their amino acid compositions.
The adult Japanese monkey (macaca fuscata fuscara) hemoglobin was converted into globin by removal of heme. The globin was separated into ƒ¿ and,ƒÀ • @ polypeptide • @ chains • @by • @ countercurrent • @ distribution • @ with • @the • @ solvent system of trichloroacetic acid containing sec-butanol, propionic acid, and water. • @ The • @ ƒ¿ • @ polypeptide • @ chain • @ was • @ hydrolyzed • @ wi...
متن کاملThe N-terminal structure of adult hemoglobin from Japanese monkey (Macaca fuscata fuscata) and the N-terminal amino acids of all the tryptic peptides from its alpha and beta polypeptide chains.
The N-terminal structure of adult Japanese monkey (macaca fuscata fuscata) hemoglobin and the N-terminal amino acid analyses of the tryptic peptides from this hemoglobin were performed. Hemoglobin, after removal of heme, was dinitrophenylated. The DNP-globin was hydrolyzed with HCl, and from the hydrolysate DNP-derivatives were extracted with ether or ethyl acetate. They were isolated and ident...
متن کاملComparative biochemistry of hemoglobins. 8. Isolation of what is called soluble tryptic peptides from the beta-polypeptide chain of Macaca mulatta monkey hemoglobin and their amino acid compositions.
Title Comparative Biochemistry of Hemoglobins VIII. Isolation of What is Called Soluble Tryptic Peptides from the β-Polypeptide Chain of Macaca mulatta Monkey Hemoglobin and their Amino Acid Compositions Author(s) Takei, Hiroshi Citation Acta Medica Nagasakiensia. 1966, 11(1-2), p.84-96 Issue Date 1966-10-25 URL http://hdl.handle.net/10069/17432 Right NAOSITE: Nagasaki University's Academic Out...
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تاریخ انتشار 2016